A microfluidic liquid phase microextraction method for drugs and parabens monitoring in human urine

Frequent consumption of pharmaceuticals and personal care products (PPCPs) have emerged as a current problem that highlights the pressing need for new multi-residue analytical methods allow their simultaneous determination to assess overall effect on human health. In this regard first time, versatile microfluidic based- liquid phase microextraction (LPME) method was developed monitoring ten compounds from six different classes: amoxicillin, sulfadiazine, sulfamerazine, tiamphenicol, ethyl 4-hydroxybenzoate, flumequine, propyl 5-hydroxydiclofenac, 3-hydroxydiclofenac diclofenac. The device combined with HPLC-UV system separation model analytes in sample. Optimal conditions were reached using 2-nitrophenyl octyl ether supported membrane, pH 3.5 donor phase, 11.5 acceptor 0.5 µL min−1 flow rate 1 rate. Under optimal conditions, extraction efficiency between 85 100% most after 10 min extraction, it successfully applied non-diluted urine, recoveries 70 all except sulfamerazine (52% recovery). addition, metabolites (3-hydroxydiclofenac 5-hydroxydiclofenac) also demonstrated systems 71 urine. proposed allowed consecutive only requires 5 organic solvent less than 15 sample volume.